Outlier Removal

The distributions of each parameter will be searched for outliers according to a method of choice and rows containing outliers will be removed.

Options

Method
The method to determine the lower and upper bounds of the data (outlier limits). Mean +- SD: This assumes a normal distribution. outliers are defined to be greater than "Mean + Factor*SD" or smaller than "Mean - Factor*SD". Factor is 3 per default. Boxplot: outliers are defined to be greater than "Q85+Factor*IQR" or smaller than "Q25 - Factor*IQR", where Q are the quantiles and IQR is the inter quantile range. Be careful the default 3 goes with the default method (Mean +- SD). A standard value for this method would be 1.5.
Factor
The factor multiplies the value describing the spread of the distribution.
Subsets
Select the columns by which the measurements should be grouped (example: plates, batches, runs...)
Constraints
The first column filter allows to select multiple columns to define groups. Rows that share the same values for all constraint columns belong to one and the same group.
Parameter
The second column filter is to select the paramters where the values have to be ckecked for outliers.
All Parameter
Per default unchecked. In this case an row is removed if it has an outlier value for at least one parameter. If you tick this checkbox, the row is only removed if all the values for all parameter are outliers.

Input Ports

Icon
data for outlier analysis

Output Ports

Icon
table with outlier rows removed.
Icon
No description for this port available.

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Installation

To use this node in KNIME, install the extension KNIME HCS Tools from the below update site following our NodePit Product and Node Installation Guide:

v5.12
Plugin provider: Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Plugin version: 5.5.0.v202511111303
On NodePit since: 2026-07-06
Last update: 2026-08-02
KNIME versions: v5.12, v5.11, v5.10, v5.9, v5.8, v5.7, v5.6, v5.5, v5.4, v5.3, v5.2, v5.1, v4.7, v4.6, v4.5, v4.4, v4.3, v4.2, v4.1, v4.0, v3.7, v3.6

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