Spot Detection

Based on Extraction of spots in biological images using multiscale products , Jean-Christophe Olivo-Marin Pattern Recognition 35 . This plugin uses currently some GPL code form Icy. To change the threshold factors using flow variables, you have to change the (2 * (n + 1) - 1)th value of the scaleConfig array. For example if you want to change threshold factor 0, you have to change value (2 * (0 + 1) - 1) = 1 of the scaleConfig, or if you want to change threshold factor 2 you have to change the (2 * (2 + 1) - 1) = 5th value in the scaleConfig array.

Options

avg method
The calculated value is used to generate the auto threshold with the formula (3 * value / 0.67) * threshold factor
configure levels
add or remove wavelet levels to determine the number of used levels
  • enable: enable the wavelet levels that should be used in the spot detection (at least one)
  • threshold factor: use this for fine tuning. The background auto threshold is multiplied by the factor. See node description, if the values should be changed using flow variables.
select dimensions
select a 2d slice for processing

Input Ports

Icon
input image with bright spots over dark background (use the inverter node if necessary)

Output Ports

Icon
bitmask of the detected spots

Views

Table Cell View

Workflows

Links

Developers

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