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BS-Seq Analysis Workflow

3-letter Mapping SNP and Methylation Level Calling 4-letter Alignment Alphabet Reduction CallingC -> TG -> AC -> TG -> ABackwardStrandLocal pairwise realignmentOUTPUT: SNPsOUTPUT:Methylation LevelsForwardStrandINPUT: Right Reads INPUT: Left ReadsINPUT: Reference FileMerger FileMerger FileMerger Casbar Four2three Four2three Four2three Four2three Razers3 Bisar File Viewer File Viewer Razers3 Sort by Position Sort by Name Input File Input File Input File 3-letter Mapping SNP and Methylation Level Calling 4-letter Alignment Alphabet Reduction CallingC -> TG -> AC -> TG -> ABackwardStrandLocal pairwise realignmentOUTPUT: SNPsOUTPUT:Methylation LevelsForwardStrandINPUT: Right Reads INPUT: Left ReadsINPUT: Reference FileMerger FileMerger FileMerger Casbar Four2three Four2three Four2three Four2three Razers3 Bisar File Viewer File Viewer Razers3 Sort by Position Sort by Name Input File Input File Input File

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