This nodes takes a string column and appends the information content of the barcode string into several new columns (e.g. library code, library plate number, project code, assay, date, replicate).
The node uses a regular expression that tries to match the barcode column. Possible barcode patterns can be set at:
KNIME->Preferences->KNIME->HCS-Tools->Barcode patterns
(?<libplatenumber>[0-9]{3})(?<projectcode>[A-z]{2})(?<date>[0-9]{6})(?<replicate>[A-z]{1})-(?<libcode>[_A-z\d]{3})(?<assay>[-_+\s\w\d]*)| Extracted metadata columns | ||||||
|---|---|---|---|---|---|---|
| Barcodes from input column | library plate number | project code | date | replicate | library code | assay |
| 001AL100914C-CPW1nMovn | 1 | AL | 100914 | C | CPW | 1nMovn |
| 006AL100914A-CPW1nMovn | 6 | AL | 100914 | A | CPW | 1nMovn |
| 001MB140502D-KBI_lowGlucose | 1 | MB | 140502 | D | KBI | _lowGlucose |
Each wanted meta information is marked as a named group and results in a new column.
Definition pattern:(?<groupname>regular expression)
| group name | column name |
|---|---|
| libplatenumber | library plate number |
| libcode | library code |
| projectcode | project code |
| concunit | unit of concentration |
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To use this node in KNIME, install the extension KNIME HCS Tools from the below update site following our NodePit Product and Node Installation Guide:
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