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differential_​analysis_​of_​protein data

Data preparation Calculation of p-values from t-test Calculation of fold changes Volcano plot with colours log-transformation of p-values and fold changes and p-value correction Histogram of original and corrected p-values Save plot as *.png file Differential analysis of protein dataThis workflow compares protein measurements of two sample groups using at-test. The resulting p-values are corrected for multiple testing andvisualized together with the fold changes in a volcano plot.For more information, please go to: https://www.ruhr-uni-bochum.de/mpc/forschung/medical_bioinformatics/bioinfraprot.html.en read in datasetlog2-trans.of intensitiest-testwrite row ID in new columnsextract group information from former row namesstart of loopover all proteinsrename columnso it is the samefor all iterationsextractp-valueextract p-value-log10(p)calculate mean per groupcalculate FCcombine p-value and FClog2(FC)Volcano plotFDR correctionassign colour to groupsdividing proteins in three colour groupshistogram of original p-valueshistogram of FDR-corrected p-valuesConvert image into table formatRender Image (adjust size, et.)Convert Image to *.pngSave Vulcano plot Image CSV Reader Math Formula Independentgroups t-test Transpose RowID String Manipulation Column ListLoop Start Column Rename(Regex) Column Filter Row Filter Math Formula GroupBy Transpose Column Filter Math Formula Column Filter Column Appender Math Formula Scatter Plot R Snippet Column Rename Color Manager Rule Engine Loop End Histogram Histogram Image To Table Renderer to Image Table To Image Image Writer (Port) Data preparation Calculation of p-values from t-test Calculation of fold changes Volcano plot with colours log-transformation of p-values and fold changes and p-value correction Histogram of original and corrected p-values Save plot as *.png file Differential analysis of protein dataThis workflow compares protein measurements of two sample groups using at-test. The resulting p-values are corrected for multiple testing andvisualized together with the fold changes in a volcano plot.For more information, please go to: https://www.ruhr-uni-bochum.de/mpc/forschung/medical_bioinformatics/bioinfraprot.html.en read in datasetlog2-trans.of intensitiest-testwrite row ID in new columnsextract group information from former row namesstart of loopover all proteinsrename columnso it is the samefor all iterationsextractp-valueextract p-value-log10(p)calculate mean per groupcalculate FCcombine p-value and FClog2(FC)Volcano plotFDR correctionassign colour to groupsdividing proteins in three colour groupshistogram of original p-valueshistogram of FDR-corrected p-valuesConvert image into table formatRender Image (adjust size, et.)Convert Image to *.pngSave Vulcano plot Image CSV Reader Math Formula Independentgroups t-test Transpose RowID String Manipulation Column ListLoop Start Column Rename(Regex) Column Filter Row Filter Math Formula GroupBy Transpose Column Filter Math Formula Column Filter Column Appender Math Formula Scatter Plot R Snippet Column Rename Color Manager Rule Engine Loop End Histogram Histogram Image To Table Renderer to Image Table To Image Image Writer (Port)

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Created by: schorkzs
Created at: 2020-01-20
On NodePit since: 2020-07-14
Last update: 2024-11-18
Created with KNIME version: v4.1.3

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