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Generic Workflow Nodes for KNIME: SeqAn version by Freie Universitaet Berlin, Universitaet Tuebingen, and the SeqAn Team

This program allows one to build a RABEMA gold standard. The input is a reference FASTA file and a perfect SAM/BAM map (e.g. created using RazerS 3 in full-sensitivity mode).

The input SAM/BAM file must be sorted by coordinate. The program will create a FASTA index file REF.fa.fai for fast random access to the reference.

Web Documentation for RabemaBuildGoldStandard


Turn this option off to disable version update notifications of the application.
Enable verbose output.
Enable even more verbose output.
Enable oracle mode. This is used for simulated data when the input SAM/BAM file gives exactly one position that is considered as the true sample position.
When set, N matches all characters without penalty.
Set distance metric. Valid values: hamming, edit. Default: edit.
Maximal error rate to build gold standard for in percent. This parameter is an integer and relative to the read length. In case of oracle mode, the error rate for the read at the sampling position is used and RATE is used as a cutoff threshold.

Input Ports

Path to load reference FASTA from. [fq,fq.bgzf,fq.gz,fastq,fastq.bgzf,fastq.gz,fa,fa.bgzf,fa.gz,fasta,fasta.bgzf,fasta.gz,faa,faa.bgzf,faa.gz,ffn,ffn.bgzf,ffn.gz,fna,fna.bgzf,fna.gz,frn,frn.bgzf,frn.gz,embl,embl.bgzf,embl.gz,gbk,gbk.bgzf,gbk.gz,raw,raw.bgzf,raw.gz,sam,sam.bgzf,sam.gz,bam]
Path to load the "perfect" SAM/BAM file from. [bam,sam,sam.bgzf,sam.gz,opt.]

Output Ports

Path to write the resulting GSI file to. [gsi,gsi.gz]


RabemaBuildGoldStandard Std Output
The text sent to standard out during the execution of RabemaBuildGoldStandard.
RabemaBuildGoldStandard Error Output
The text sent to standard error during the execution of RabemaBuildGoldStandard. (If it appears in gray, it's the output of a previously failing run which is preserved for your trouble shooting.)

Best Friends (Incoming)

Best Friends (Outgoing)



To use this node in KNIME, install SeqAn from the following update site:


A zipped version of the software site can be downloaded here.

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