HCS-Tools for KNIME version 4.0.0.v201906200802 by Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Each plate will be normalized by applying the B score. The B score algorithm uses an iterative 'median polish' procedure.
In brief, the algorithm uses robust plate, row and column estimates to eliminate plate, row and column position biases that result e.g. from egde effects.
Literature: Brideau et al., J Biomol Screen 2003; 8; 634, Tukey JW, Exploratory Data Analysis. Reading (MA): Addison-Wesley, 1977.
Note: The node expects the presence of integer columns named 'plateRow' and 'plateColumn' containing the well position.
To use this node in KNIME, install KNIME HCS Tools from the following update site:
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