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Channel correction

Detect spots Measure transformation Apply transformation View results Read ch1 bead imageRead ch2 bead imageCorrectimagesCorrectpoint coordinatesRename{x,y,z}Out -> i{x,y,z}1 Image Reader Image Reader Spot Detection (Subpixellocalization) Spot Detection (Subpixellocalization) Measure 3D TransformationBetween Spots Sanitize Row ID Sanitize Row ID Joiner Joiner Apply 3D AffineTransform Apply 3D AffineTransform to Points Unpivot Vector Data Column Filter Joiner Unpivot Vector Data Joiner Merger Image Viewer R View (Table) Column Rename(Regex) R View (Table) Detect spots Measure transformation Apply transformation View results Read ch1 bead imageRead ch2 bead imageCorrectimagesCorrectpoint coordinatesRename{x,y,z}Out -> i{x,y,z}1Image Reader Image Reader Spot Detection (Subpixellocalization) Spot Detection (Subpixellocalization) Measure 3D TransformationBetween Spots Sanitize Row ID Sanitize Row ID Joiner Joiner Apply 3D AffineTransform Apply 3D AffineTransform to Points Unpivot Vector Data Column Filter Joiner Unpivot Vector Data Joiner Merger Image Viewer R View (Table) Column Rename(Regex) R View (Table)

Nodes

Extensions

Links

Download

To use this workflow in KNIME, download it from the below URL and open it in KNIME:

Download Workflow
Created by: Jan Eglinger
Created at: 2019-04-02
On NodePit since: 2019-04-29
Last update: 2024-04-25
Created with KNIME version: v3.7.1
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