## Description
OpenMS exam workflow for the analysis of isobaric data (e.g. TMT).
A) Quantification via the IsobaricAnalzyer
B) Score estimation and filtering (PSM level)
C) Database search and annotation (target-decoy)
D) Merging of the results
E) Score estimation and protein inference (epifany)
F) Peptide level results
G) Protein level results
H) MSstatsTMT output (peptide level - experimental)
## Input
1) Input files (isobaric labeled peptides (e.g. TMT) as centroided mzML files.
2) Decoy database (.fasta)
3) Experimental Design (.tsv) for MSstatsTMT
## Output
1) MSstatsTMT readble format (.tsv)
2) Peptide level information (.mztab)
3) Protein level information (.csv/mztab)
Example input data:
To download the data from the archive linked below, check the box on the folder and click on the download button (arrow) on the top left.
Download and unpack the "Example_data" data into a directory "Example_data" inside your KNIME workspace.
To use this workflow in KNIME, download it from the below URL and open it in KNIME:
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