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PileupCounts

DeprecatedNGS related nodes for KNIME Workbench version 0.2.200.qualifier by Bernd Jagla, Institute Pasteur

PileupCounts counts occurences of e.g. matched positions from the corresponding from the mpileup command from samtools Here is the description of the mpileup command from samtools: Generate BCF or pileup for one or multiple BAM files. Alignment records are grouped by sample identifiers in @RG header lines. If sample identifiers are absent, each input file is regarded as one sample. In the pileup format (without -uor-g), each line represents a genomic position, consisting of chromosome name, coordinate, reference base, read bases, read qualities and alignment mapping qualities. Information on match, mismatch, indel, strand, mapping quality and start and end of a read are all encoded at the read base column. At this column, a dot stands for a match to the reference base on the forward strand, a comma for a match on the reverse strand, a '.gt.' or '.lth.' for a reference skip, 'ACGTN' for a mismatch on the forward strand and 'acgtn' for a mismatch on the reverse strand. A pattern '\+[0-9]+[ACGTNacgtn]+' indicates there is an insertion between this reference position and the next reference position. The length of the insertion is given by the integer in the pattern, followed by the inserted sequence. Similarly, a pattern '[0-9]+[ACGTNacgtn]+' represents a deletion from the reference. The deleted bases will be presented as '*' in the following lines. Also at the read base column, a symbol '^' marks the start of a read. The ASCII of the character following '^' minus 33 gives the mapping quality. A symbol '$' marks the end of a read segment.

Options

pileup column
column with pattern

Input Ports

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Pileup file from samtools usning mpileup

Output Ports

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added columns with how many mismatches etc occred

Installation

To use this node in KNIME, install KNIME NGS tools from the following update site:

KNIME 4.3

A zipped version of the software site can be downloaded here.

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Developers

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