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HCS-Tools for KNIME version 4.0.0.v202007151505 by Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

The coefficient of variation (CV), also known as relative standard deviation (RSD), is a standardized measure of dispersion of a probability distribution.

For each group of data (e.g per plate), CVs are calculated for each selected subset (e.g. control wells of a plate).

Original CV = sd(x[subset] / mean(x[subset]) * 100
Robust CV = mad(x[subset] / median(x[subset]) * 100

Wikipedia: Coefficient of variation


General Settings

Group wells by
Select the column to define the main groups (e.g. "barcode" for plate-wise CVs).
Select subset column / well annotation column
Select the column to define and select subsets (e.g. "treatment" for controls )
Subset selection available in tab "Subset Filter".
Use robust statistics
If checked median and MAD will be calculated instead of mean and standard deviation.
Calculate coefficient of variation for
Select the numeric columns for which CVs will be calculated.
Column suffix
By default result column names will get the suffix ".cv". If checked, this suffix can be changed or removed.

Subset Filter

Include subsets the CV shall be calculated for. Missing values can be included via checkbox.

Input Ports

Input Data

Output Ports

Table with CVs

Best Friends (Incoming)

Best Friends (Outgoing)



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A zipped version of the software site can be downloaded here.

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