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Investigating parasite recovery from translation inhibition

Exploratory analysis of acute translation inhibition and recovery in singlePlasmodium berghei liver stage parasites. The data included here were used to generate Fig. 2-3 and associated supplemental figures for:McLellan, J. L., & Hanson, K. K. (2023). Translation inhibition efficacy does not determine the Plasmodium berghei liverstage antiplasmodial efficacy of protein synthesis inhibitors. bioRxiv, 2023.2012.2007.570699.doi:10.1101/2023.12.07.570699 Interactive data sub setting to explore single cell data via subsetting compound treatment, timepoint, and experimental replicateusing a scatter plot viewer. To view, right click node and select "interactive view." Interactive comparisons between translation inhibition classes toexplore the relationship between translation inhibition efficacy onparasite size and growth during an acute treatment, and aftercompound washout.To view, right click node and select "interactive view." Individual parasites with atranslation intensity < 50% ofcontrol parasites at 28 hpi wereclassified as translation impaired.This impairment classification wasused as an arbitrary cutoff toinvestigate the relationship betweenparasite size and translation. This"inhibitied" classification can bechanged inside the metanode todifferent values, or altered to querythe dataset as desired. This workflow and the underlying data was generated in the Hanson Lab in the Department ofMolecular Microbiology and Immunology and STCEID at the University of Texas at San Antonio.Questions about the scientific data should be directed to Kirsten Hanson(kirsten.hanson@utsa.edu). Questions about the workflow can be directed to either JamesMcLellan (james.mclellan@utsa.edu) or Kirsten Hanson. Node 4733 Interactive ScatterPlots Dashboard Compound-concentrationjitter generated. READ ME Change Log Classification of translationimpaired parasites Interactive ViolinPlots Dashboard CSV Reader Exploratory analysis of acute translation inhibition and recovery in singlePlasmodium berghei liver stage parasites. The data included here were used to generate Fig. 2-3 and associated supplemental figures for:McLellan, J. L., & Hanson, K. K. (2023). Translation inhibition efficacy does not determine the Plasmodium berghei liverstage antiplasmodial efficacy of protein synthesis inhibitors. bioRxiv, 2023.2012.2007.570699.doi:10.1101/2023.12.07.570699 Interactive data sub setting to explore single cell data via subsetting compound treatment, timepoint, and experimental replicateusing a scatter plot viewer. To view, right click node and select "interactive view." Interactive comparisons between translation inhibition classes toexplore the relationship between translation inhibition efficacy onparasite size and growth during an acute treatment, and aftercompound washout.To view, right click node and select "interactive view." Individual parasites with atranslation intensity < 50% ofcontrol parasites at 28 hpi wereclassified as translation impaired.This impairment classification wasused as an arbitrary cutoff toinvestigate the relationship betweenparasite size and translation. This"inhibitied" classification can bechanged inside the metanode todifferent values, or altered to querythe dataset as desired. This workflow and the underlying data was generated in the Hanson Lab in the Department ofMolecular Microbiology and Immunology and STCEID at the University of Texas at San Antonio.Questions about the scientific data should be directed to Kirsten Hanson(kirsten.hanson@utsa.edu). Questions about the workflow can be directed to either JamesMcLellan (james.mclellan@utsa.edu) or Kirsten Hanson. Node 4733 Interactive ScatterPlots Dashboard Compound-concentrationjitter generated. READ ME Change Log Classification of translationimpaired parasites Interactive ViolinPlots Dashboard CSV Reader

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Created by: kkh
Created at: 2023-12-01
On NodePit since: 2023-12-06
Last update: 2024-04-27
Created with KNIME version: v4.5.1

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