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Normalizer

MassSpectInKnime2 version 1.0.1

This node is useful for normalizing mass spectrometry intensities. It supports 3 methods of normalization.

Options

TIC
This is the industry standard in normalization of MSI data, specifically, it calculates the Total Ion Count, ie the sum of the intensities. Then we divide each intensity in that spectrum by its TIC.
Median
This method of normalization is simple and is gaining popularity in MSI. We simply calculate the median intensity for a spectrum. Then we divide each intensity value by this.
Euclidean Norm
This method of Normalization involves calculating the Euclidean Norm of the intensities of our Mass spectrometry data. Then we divide each intensity value by this.

Input Ports

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The incoming data should contain rows of data that are to be independently normalized.

Output Ports

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The outgoing data consists of the normalized incoming data.

Installation

To use this node in KNIME, download the below referenced file, save it to your KNIME's plugin folder and restart KNIME.

KNIME 4.3

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Developers

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