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01_​Spot_​Detection

Application: Spot Detection
Pre-Processing There are various otherways to preprocess yourdata. Search for filters inour node-repository Spot-Detection: Difference of Gaussians Spot-Detection: Laplacian of Gaussians Spot-Detection: More variants This tutorial workflow shows some very basic approaches for spot-detection in images. General InformationClick execute to run the complete workflow. You may have to scroll down to see all contents of this tutorial.Furthermore, you may change some connections to see the behaviour of the other variants, e.g. in the Pre-Processing or Spot-Detection.To see the contents of a metanode (gray nodes), double-click it. This opens a new tab showing you the nodescontained in this metanode.Workflow InformationThis tutorial workflow shows some very basic approaches for spot-detection in images. The power lies in thecombination of the various methods. Note that you will need to adjust the parameters to get nice results that suityour respective image data.PrerequisitesIn order to understand this tutorial we suggest that you also look at the following:Difference_of_GaussiansBasic Node TutorialsIf you need more information about other topics and more tutorials, see:http://tech.knime.org/community/image-processingIf you have any questions please contact us via our forum:http://tech.knime.org/forum/knime-image-processing/Workflow RequirementsKNIME 3.1.2KNIME Image Processing 1.4.2 Sigma FilteringDoG ImgLib2 ImplementationConverting to ByteTypeNormalize the imgAdvanced Laplacian of GaussianAdvanced DoGManual - Not Very robustA little better than global thresholderNice Spot-Detection algorithm Gaussian Convolution(deprecated) Quantile Filter Sigma Filter Max Homogeneity DoG Spot Detection(deprecated) Image Converter Image Normalizer Advanced LoG Advanced DoG Global Thresholder Local Thresholding Spot Detection Spot-Postprocessing Image Reader Pre-Processing There are various otherways to preprocess yourdata. Search for filters inour node-repository Spot-Detection: Difference of Gaussians Spot-Detection: Laplacian of Gaussians Spot-Detection: More variants This tutorial workflow shows some very basic approaches for spot-detection in images. General InformationClick execute to run the complete workflow. You may have to scroll down to see all contents of this tutorial.Furthermore, you may change some connections to see the behaviour of the other variants, e.g. in the Pre-Processing or Spot-Detection.To see the contents of a metanode (gray nodes), double-click it. This opens a new tab showing you the nodescontained in this metanode.Workflow InformationThis tutorial workflow shows some very basic approaches for spot-detection in images. The power lies in thecombination of the various methods. Note that you will need to adjust the parameters to get nice results that suityour respective image data.PrerequisitesIn order to understand this tutorial we suggest that you also look at the following:Difference_of_GaussiansBasic Node TutorialsIf you need more information about other topics and more tutorials, see:http://tech.knime.org/community/image-processingIf you have any questions please contact us via our forum:http://tech.knime.org/forum/knime-image-processing/Workflow RequirementsKNIME 3.1.2KNIME Image Processing 1.4.2 Sigma FilteringDoG ImgLib2 ImplementationConverting to ByteTypeNormalize the imgAdvanced Laplacian of GaussianAdvanced DoGManual - Not Very robustA little better than global thresholderNice Spot-Detection algorithm Gaussian Convolution(deprecated) Quantile Filter Sigma Filter Max Homogeneity DoG Spot Detection(deprecated) Image Converter Image Normalizer Advanced LoG Advanced DoG Global Thresholder Local Thresholding Spot Detection Spot-Postprocessing Image Reader

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